Surface Sanitation Verification

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Submitted By eireone
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SURFACE SANITATION VERIFICATION
PURPOSE:
The primary purpose of this information paper is to provide guidance on the use and implementation of the Charm PocketSwab( for surface sanitation verification in commissaries located throughout EUCOM.
BACKGROUND:
ATP (adenosine triphosphate) bioluminescence has been used in the food industry for over 10 years to quickly assess and monitor microbial contamination on surfaces. ATP is an excellent means of identifying “hot spots” or areas with organic material that could possibly support and accelerate microbial growth. The technology of ATP bioluminescence for hygiene monitoring has become increasingly useful because of it real-time capabilities, ease of use and affordability. It cannot replace microbiological testing but it is easier, faster and the results indicate cleanliness, (defined as the absence of organic material), rather than counting colony forming units of microorganisms.
SCIENTIFIC PRINCIPLE:
It is important to understand the basic principles of ATP bioluminescence. ATP is a molecule that is essential and common to all plant, animal and microbial cells. This compound combines and reacts with an enzyme (luciferase) resulting in the release of light. The light emitted is measured by using the Charm LUM-T. This output of light is proportional to the amount of ATP present on a given surface. The measurement of biological contamination is approximated by determining the amount of ATP contained within or on the material. The sample collected is typically a 4-inch square area (100 cm2) that has been swabbed with a PocketSwab(. For reproducibility and consistency from one inspector to the next, it is imperative that the surface area covered with the swab is standardized. The ATP is then extracted from the swab material and mixed with luciferin/luciferase. It is the combination between the luciferase and…...

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