Bio 362 Exam Iii Solution 100% Correct Answers

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BIO 362 Exam III Solution 100% Correct Answers
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Question 1. (30 pts)
Given the following DNA template strand of reporter gene (a gene used to report on mRNA and protein expression):
5-CTATACTCCTCCATGTCTTACGTGGTAGTGGTGGTAGTGTACATCAAAAAAAAA-3
a) Write the likely mRNA sequence of this gene when it is expressed in bacteria. (2 points)
b) Write the anticodon sequence of all tRNAs that could decode this mRNA (without Inosine modifications in the tRNAs). Please make sure to indicate the correct directionality of the codons and anticodons. (4 points)
c) What is the minimum number of tRNAs required to decode this mRNA? Explain your answer. (2 points)
1
Bio362-2013
Exam III
Name
d) Write the likely amino acids sequence of the protein produced from this gene (2 points)
e) Which approach/technique will you use to detect this mRNA? Briefly explain how this approach works and what material/reagents you will need. (2 points)
f) Which approach/technique will you use to detect this protein? Briefly explain how this approach works and what material/reagents you will need. (3 points)
2
g) You have discovered a new viral protein called Snoop that is a sequence specific mRNA modification enzyme. When you express Snoop in bacteria or eukaryotes, along with your reporter gene (shown above), you notice that you are unable to detect the reporter mRNA or its protein product.
i.
Explain why there is no detectable reporter mRNA or protein when Snoop is expressed in bacterial. (5…...

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